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Spectral fluorometric characterization of Haptophyte dynamics using the FluoroProbe: an application in the eastern English Channel for monitoring Phaeocystis globosa ArchiMer
Houliez, Emilie; Lizon, Fabrice; Thyssen, Melilotus; Artigas, Luis Felipe; Schmitt, Francois G..
In this study, we examined the possibility of using the FluoroProbe for monitoring the dynamics of the Haptophyte Phaeocystis globosa in the coastal waters of the eastern English Channel. The FluoroProbe was recalibrated by recording a new fingerprint for P. globosa and the use of this new fingerprint was tested through a series of laboratory and in situ experiments. The annual dynamics of P. globosa estimated using the FluoroProbe and by flow cytometry were similar. A strong relationship was found between the FluoroProbe estimates of P. globosa biomass expressed in terms of chlorophyll a equivalent per litre (eq. mu g L(-1)) and flow cytometric cell counts (r = 0.889, P < 0.001, n = 121). The FluoroProbe can be used to detect the flagellated cells as...
Tipo: Text Palavras-chave: Spectral fluorescence; Phaeocystis globosa; Haptophytes; Algal blooms; Phytoplankton quantification; Chlorophyll a.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00373/48461/48723.pdf
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Transcription factors in microalgae: genome-wide prediction and comparative analysis ArchiMer
Thiriet-rupert, Stanislas; Carrier, Gregory; Chenais, Benoit; Trottier, Camille; Bougaran, Gael; Cadoret, Jean-paul; Schoefs, Benoit; Saint-jean, Bruno.
Background: Studying transcription factors, which are some of the key players in gene expression, is of outstanding interest for the investigation of the evolutionary history of organisms through lineage-specific features. In this study we performed the first genome-wide TF identification and comparison between haptophytes and other algal lineages. Results: For TF identification and classification, we created a comprehensive pipeline using a combination of BLAST, HMMER and InterProScan software. The accuracy evaluation of the pipeline shows its applicability for every alga, plant and cyanobacterium, with very good PPV and sensitivity. This pipeline allowed us to identify and classified the transcription factor complement of the three haptophytes...
Tipo: Text Palavras-chave: Algae; Endosymbiotic gene transfer; Haptophytes; Prediction pipeline; Stramenopiles; Tisochrysis lutea; Transcription factors.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00333/44403/44072.pdf
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